NEU259: Introduction to Light and Electron Microscopy (Fall 2014)
Tuesday/Thursday 10:00 am - 11:00 am (Room 1000 BSB )
Demos Thursday 11:00 am- 12:30 pm (TBA)
| Gina Sosinsky, Ph.D gsosinsky@ucsd.edu X40128 |
Mark Ellisman, Ph.D. mellisman@ucsd.edu X42251 |
TA: Nathaniel Wood-Cohan nwoodcoh@ucsd.edu X44583 |
Recommended readings will be per individual instructors.Reading materials on electron microscopy can be found at http://cryoem.ucsd.edu/courses or posted to a Dropbox class area. Password for pdf downloads will be announced at first class or contact Dr. Sosinsky.
Our course websites can be accessed at http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm
Syllabus: (document download)
No |
Date |
Lecture |
Lecturer |
Demo |
1 |
2-Oct Th |
Intro to Course / Intro to Optics I |
Gina Sosinsky/ James Bouwer |
NCMIR Tour-ME |
2 |
7-Oct Tu |
Intro to Optics II |
James Bouwer |
|
3 |
9-Oct Th |
Confocal Microscopy /Fluorescence |
Tom Deerinck |
LM - HH/GS |
4 |
14-Oct Tu |
Multiphoton Microscopy |
James Bouwer |
|
5 |
16-Oct Th |
Transmission Electron Microscopy |
Tom Deerinck |
TEM – TD |
6 |
21-Oct Tu |
Specimen Preparation |
Gina Sosinsky |
|
7 |
23-Oct Th |
Immunocytochemistry for LM and EM |
Gina Sosinsky |
Confocal – |
8 |
28-Oct Tu |
In Situ Hybridization |
Angela Cone |
|
9 |
30-Oct Th |
Optical probes for live cell imaging |
Varda Lev-Ram |
SEM – TD |
10 |
4-Nov Tu |
Midterm |
|
Midterm extra time |
11 |
6-Nov Th |
Advanced Light Microscopy Techniques (e.g. TIRF, PALM, STED, etc.) |
Hiro Hakozaki |
|
|
11-Nov Tu |
Veterans Day Holiday |
******* |
******* |
12 |
13-Nov Th |
Digital Imaging |
James Bouwer |
Common Image Pro- |
13 |
18-Nov Tu |
Image Processing/Analysis Fundamentals |
Gina Sosinsky |
|
14 |
20-Nov Th |
Deconvolution Microscopy |
James Bouwer |
|
15 |
25-Nov Tu |
Scanning Probe Microscopies |
Gina Sosinsky |
AFM demo- tentative |
|
27-Nov Th |
Thanksgiving Holiday Week |
******* |
******* |
16 |
2-Dec Tu |
Electron Tomography |
Guy Perkins |
|
17 |
4-Dec Th |
Databases and Image Informatics |
Maryann Martone |
Tomography |
18 |
9-Dec Tu |
Molecular Microscopy: Specimen Prep |
Gina Sosinsky |
|
19* |
11-DecTh |
Molecular Microscopy: 3D Recon Methods |
Tim Baker |
TBD |
20 |
*********** |
**Finals Week Dec.15-19 |
********************* |
|
* Written assignment “My thesis project and how microscopy can contribute to my thesis”. (1 page limit is for the body of writing. Use Arial font, 11 pt, 1 inch margins (2.5 cm) relative to 8.5 x 11 inch paper format. Line spacing should be 6 lines/inch (use single spacing with Microsoft Word).
** Final examination is take-home and due back by Dec 18th (last Thursday in the quarter).
