NEU260: Workshop in Light and Electron Microscopy (Fall 2014)

Tuesday/Thursday 1:00 pm - 3:30 am (Room 1000 BSB )

Gina Sosinsky, Ph.D

gsosinsky@ucsd.edu

X40128		 Mark Ellisman, Ph.D.

mellisman@ucsd.edu

X42251		 TA: Nathaniel Wood-Cohan

nwoodcoh@ucsd.edu

X44583


Recommended readings will be per individual instructors.Reading materials on electron microscopy can be found at http://cryoem.ucsd.edu/courses or posted to a Dropbox class area. Password for pdf downloads will be announced at first class or contact Dr. Sosinsky.

Our course websites can be accessed at http://ncmir.ucsd.edu/training/ucsd_courses/index.shtm

Syllabus: (document download)

NOTE: TEXT IN BLUE REFERS TO CLASSES SHARED BY ALL STUDENTS

Lab # Date

 EM Schedule

 Instructors

 LM Schedule

 Instructors
1 2-Oct Th Orientation Sosinsky, Ellisman, Deerinck, Bushong, Hakozaki, Mackey, Wood-Cohan
2 7-Oct Tu Immuno

 Bushong / Cone
3 9-Oct Th

Microtomy

 Mackey / Bushong / Kim / Deerinck
4 14-Oct Tu Specimen Prep Fixation Mackey / Bushong / TBN
5

 16-Oct Th Meet for project setup Sosinsky, Ellisman, Deerinck, Bushong, Hakozaki, Mackey, Wood-Cohen
6 21-Oct Tu LM imaging basics Hakozaki EM imaging basics Mackey/ TBN
7 23-Oct Th EM I Mackey / TBN LM I Hakozaki
8 28-Oct Tu Embedding/Knife-making Mackey / TBN LM II Hakozaki
9 30-Oct Th EM II Mackey Live Cell Imaging Cone
10 4-Nov Tu EM Alignment Mackey Advanced LM Alignment Hakozaki
11 6-Nov Th Practice and Project Time
  11-Nov Tu Veterans Day Holiday
12 13-Nov Th Electron Tomography I (Experimental)

Perkins, Mackey

 Practice/Project Time Hakozaki, TBD
13 18-Nov Tu Electron Tomography II (Computational) Perkins FRET/FRAP Hakozaki, Hoshijima
14 20-Nov Th EM Practical Training HPF-FS demo

Mackey

Thor (HPF)

 Deconvolution/ TIRF Hakozaki/Bushong
15 25-Nov Tu Project Time and Written Exams Mackey or Hakozaki
  27-Nov Th Thanksgiving Holiday
16 31-Nov Tu Visualization Deerinck, Bushong, Sosinsky
17 4-Dec Th Project Time Mackey or Hakozaki
18 9-Dec Tu Project Time & Skills Assessment Mackey or Hakozaki
Finals week 18-Dec Thu Student Presentations (at 11:30 am) All instructors come*
** for those taking Neu259, Neu259 exams will be due Friday Dec 19.

 

 

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